RAW2647小鼠巨噬细胞protocol.docx

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1、RW264.7细胞株的相关信息RAW2e4.7nU$431.0My：ATCCMtte及太费图：5W*!H三H索取报价VAccmw三y：*ItS人：ATCC啪由工电懦：1800638659T如伴:onafleei8三e三tt5JUff0TIi随计划让您仍为加心|XDSign徜onsRAW网7Dpo4orWCRwchM,BcateWShjmcBA1.BkIbwcsuitesDtseMe:Abetonmunneleukemiavrus11edtumorCeiIyp:mtcroaoe.AbelsonrrMrbewuemsirttwr/TrnfrdhrsccymS*WonIo9MTAme11noneea

2、bove,otherATCCanorrwWvw114sEbeRWMforMetansferofPmMtFon8TATCCmrenAfrronepurchasingATCm秋ggmwyrgwns?forowningtheerme;PACtoCkFwfQVrmanonrg*dngZwcifkWMrRmeftSforSNprrwnItoVWlocalsBoloaiCdiresponseApplkauonsYansracaonhotSoIUf8CeHuUfProductsReccplorsAntanEnxeseion.Me.CarWWcomplemr(C3)1207H-MadultmaAComment

3、sSubculhJfingReuiedProductsFor-Profit:$?Non-Profit:12Q1JAddtoCaitThislinewasWUmh，gaWfWnJc6AhelsonmurtnejgmuWUftTMy第。n0阿2IeWSUCCgImmuncfllobulin).la0andThyI2(Tfrr12)ThislinedoesnotsecretedetectablevrsPartCH3andnnegate讷meCoeft)rmonassayTheCeU*iOQgIOsnr伯GanoaoocbeadsandrmosanTheyamcapablef4ntb0d/depfM3

4、ergiOfShMOeMgsWtumorelAgffK1.PSorPPDtealmentftx2daytt3rrMateshrwsoffMroc4wbutno!tumorcelUrgeisD3Ocomunca!edInFg2007OrJanetWKirtey.ncsrw11wcnofnfe(8usecotropicMU1.Va9，觥皿r110te11)cMtotcMo1911vl*vhelperwususedintheCngmaWU$InOCUUmTocellsKsocipresspoFoWu1.V1guhsif19&basedonZmoupa99冷hisk*rctney11slocks(Pu

5、t*d1S1775OCATCCcM11tetgrowthIiwdMNncThebasemediumfcf9HscelllineaATCC-VmuIatodDb4ccoMc3iodEeMeaUmtCataBNo30-2002Tomatethecompletegr*1medum.ac311dlo)doC02),5%UnpeU*37.(TCPTotSuuuIMGft3f980呷90”卬WngFora75cm2ft*sk,rem(wtbut!Omlculturemedium(adustmourrtaccorntftorctercuturevssels)D8c*wcesfromtneflasksubst

6、rate*ia(蝴Mfaperaspiratend*appwiea04sofme(剖suspensioninto11o,*cubJf8v*MSubcuIlKMtKmMio:ANbCUESr10011.3o1.6neomrrndIMtalmRmiawMRgploceOfadomumsery23darr$freezermum:CcmpUOeQrm0umsupplemcrtcdwl6%C)DMSO袁十*itMN:quidMrOoenvaporphaseRecommended11wum(VtOhouttheaddrtonaiSUPQtemonnCfMrUmdscnt43underATCCMeUrn)A

7、TCC302002faomfw3MServnATCC30-20201135Raqp*iP.NakorcIArUDotffdependantMlingofo11trc951.1977PubMW8940311207Rhk*WC.etFuncnonalmcrodesrum(DMM)motetocontain4rrM1.-gM&mtne.4500rrgf1.gfcose,1rrMSOdiumpyruvalefsdiXOmgf1.sodiumbicatm那.Formulation:Fam3灰IOnProductFormat:500m1.Comments:NOTE:This/educedfevHofsdf

8、umbica,bomg(NHCO3,1.5gf1.)isintendedforUSein5%CO2inair.AddHionsisodiumbicarbon*QmayberequitedforuseinncbtorscontainingigherpercentagesofCO2SubculturingProcedureSubculturesarepreparedbyscrapingFora75cm2flask,removeallbut10mlculturemedium(adjustamountaccordinglyforotherculturevessels).Dislodgecellsfro

9、mtheflasksubstratewithacellscraper;aspirateandaddappropriatealiquotsofthecellsuspensionintonevculturevessels.SubcultjvationRatio:1:3to1:6MediumRenewalReplaceoraddmediumevery2to3days.CompleteGrowthMediumThebasemediumforthiscelllineisATCC-formulatedDulbeccosModifiedEaglesMedium.CatalogNo.30-2002.Tomak

10、ethecompletegrowthmedium,addthefollowingcomponentstothebasemedium:fetalbovineserumtoafinalconcentrationof10%Thismediumisformulatedforusewitha5%CO2inairatmosphere.(StandardDMEMformulationscontain3.7g/1.sodiumbicarbonateanda10%CO2inairatmosphereisthenrecommended).ATCCtestedfetalbovineserumisavailablea

11、sATCCCatalogNo.30-2020andATCCCatalogNo.30-2021(100ml).CryoprotectantMediumCompletegrowthmediumdescribedabovesupplementedwith5%(vv)DMSO.ATCC：美国模式培育物集存库(AmeriCantypeculturecollection)的简写收到冷冻管细胞的处理方式1.收到细胞株包袱时，请检查细胞株冷冻笆是含有解冻情形.心清勺上通知.细胞株请尽速起先培杼，或马上冷冻保存（置干-70C,隔夜后,移到液氮）。2.收到T25flask细胞的处理方式于寄送过程中，为避开起泡造成细咆脱落死亡，T25flask均加满培1. 1.检查flask外观，并于显微境下视察细胞生长状况和有无污染现象，若有任何问遨.不要打开装子，请马上通知细胞试脸室.2. 2.将原财之T25flask静次于379,5%CQ2培Ff箱中，使细胞回温至37C,并让运输过程中少数脱落的细胞可再附着生长.2.3.隔天后,于无菌操作箱内取出flask内之培育基.（取出之培育基可以再运用）,仅留妁5-10l培闫基于flask内.依-般培H方式再将细胞谀入培育和中，或细胞已长满盘，则将细胞做传代培育,RAW