常用缓冲溶液的配制.docx

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1、常用缓冲溶液的配制方法1 .甘氨酸-盐酸缓冲液(0.05mo1./1.)X亳升0.2IQOIz1.甘氨酸+Y毫升0.2mo1./1.HCI,再加水稀释至200亳升PHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分上量75.07,0.2mo1./1.甘氨酸溶液含15.01克/升。2 .邻苯二甲酸-盐酸缓冲液(0.05mo1./1.)X亳升0.2bo11.邻苯二甲酸氢钾+0.2mo1./1.HC1.,再加水稀释到20亳升pH(20C)XYpH(20c)XY2.254.0703.251.4

2、70PH钠离子浓柠檬氢氧盐酸最终体积度酸（克）化钠（克）（毫升）（升严mo1.)CkHhO7NaOHHC1.H2O97%（浓）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610运用时可以每升中加入1克克酚，若最终PH值有改变，再用少量5质氢氧化钠溶液或浓盐酸调整，冰箱保存。5.柠檬酸-柠椽酸钠谖冲液（0.1mo1.1.）H0.1mo1.0.1mo1.H0.1mo1./1.0.1mo1./1.6.050.57

3、07.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液（18C）PH0.04M巴比妥钠溶液0.2V盐酸PH0.04M巴比妥钠溶液（亳升）0.2N盐酸（毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.08.21001009.397.219.61001000.35巴

4、比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10 .TriS-盐酸缓冲液(0.05M,25C)50亳升0.IM三羟甲基氨基甲烷(Tris)溶液及X毫升OjN盐酸混匀后，加水稀释至100亳升。PHX(邕升)PHX(亳升)7.1045.78.1026.27.2044.78.2022.919.97.3043.48.3017.27.4042.08.4014.77.5040.38.5012.47.6038.58.6010.37.7036.68.708.57.8034.58.807.07.9032.08.908.0029.2三羟甲基氨基甲烷（Tris）H0CH2CH20HH0CH2NH2分

5、子量=121.14;O.IM溶液为12.114克/升。Tris溶液可从空气中汲取二氧化碳，运用时留意将瓶盖严。11 .硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M硼砂（亳升）0.2M硼砂（亳升）PH0.05M硼砂（亳升）0.2M硼酸（亳升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B1O7-H2O,分子量=381.43:0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H,BO,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必需在带塞的瓶中保存。1

6、1 .硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M硼砂（亮升）0.2M硼酸（亮升）PH0.05M硼砂（亳升）0.2M硼酸（亮升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBO；IOHA分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BOs,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必需在带塞的瓶中保存。12 .甘氨酸-氢弱化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y老升0.2Nna0H加水稀释至200名升PHXYP

7、HXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.15038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13 .硼砂-氢氧化钠缓冲液(0.05M硼酸根)X空升0.05M碉砂+Y亳升0.2NNa01.1.加水稀释至200亳升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂Na2BAIOH2O,分子量=381.43。05M溶液为19.07克/升。14 .碳酸钠-碳酸氢钠缓冲液(0

8、.1M)Ca、Mg?.存在时不得运用PH0.IMNa2CO1(0.IMNzHCO：)(20C37,C亳升）亳升）9.168.77199.409.12289.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791Na2CO2IOH2O分子量=286.2;0.IV溶液为28.62克/升。NzHCO,分子量=84.0;0.IM溶液为8.40克/升。15 .“PBS”缓冲液PH7.67.47.27.0IkO100010001000100m1.NaC1.8.58.58.58.5gNa,HPO1.2.22.22.22.2gNa1.I2PO1O.10.20.3O.4g